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| Ohmori, Takashi; Tamahara, Satoshi; Thi, Pham Ngoc; Tomihari, Mizuki; Shimada, Terumasa; Matsuki, Naoaki; Ono, Kenichiro. |
| Interleukin-12 (IL-12) is one of the most important factors for the host’s protective immune response against protozoan infection. To evaluate the effect of bioactive form of IL-12(IL-12p70), expression of IFN-AmRNA and IL-4 mRNA in splenic CD4 positive T cells, anti-parasite delayed type hypersensitivity (DTH), and serum and splenic IL-12p70 concentration were examined in Babesia microti and B. rodhaini infected mice. The expression of IFN-AmRNA as a marker of Th1 cell differentiation was detected in splenic CD4 positive T cells in B. microti infected mice. Activation of DTH response as a marker of cell-mediated immune response was also observed from 2 days after the infection with B. microti, while no response was detected in B. rodhaini infected mice.... |
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Palavras-chave: Babesia microti; Babesia rodhaini; Cell-mediated immunity; Early phase of infection; IL-12p70; Th1 cell differentiation. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/150 |
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| Hashiguchi-Kato, Rie; Thi, Pham Ngoc; Ohmori, Takashi; Tamahara, Satoshi; Katayama, Shinsuke; Shimada, Terumasa; Matsuki, Naoaki; Ono, Kenichiro. |
| Stimulatory factors for interleukin 12 (IL-12) production associated with Babesia microti and Babesia rodhaini infected erythrocytes were examined using an in vitro assay system established, since a remarkable increase of serum IL-12 concentration and differentiation of helper T cell (Th cell) into helper T cell type 1 (Th1 cell) was observed in early phase of infection with Babesia spp. in mice. To investigate direct stimulating activity of infected erythrocytes for IL-12 production, intact splenic macrophages were co-cultured with them and examined the expression of IL-12 mRNA by reverse transcription-polymerase chain reaction (RT-PCR). Both B. microti and B. rodhaini infected erythrocytes elicited IL-12 mRNA expression in co-cultured macrophages. Since... |
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Palavras-chave: Babesi microti and Babesia rodhaini infected erythrocyte; IL-12 Production; Mice; Splenic Macrophage; Stimulatory factor. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/151 |
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